In vitro callogenesis essays to induce somatic embryogenesis of the Tunisian chickpea

The present study was the first report on somatic embryogenesis of Tunisian chickpea (Cicer arietinum L.) particularly supposed to be recalcitrant and difficult manipulate in vitro. An efficient protocol has been developed for inducing indirect derived from immature zygotic embryo axis, young leaflet hypocotylar explants. Callogenesis achieved full-strength Murashige Skoog's (1962) (MS) basal medium supplemented with two auxin/cytokinin combinations; 2,4-dichlorophenoxyacetic acid (2,4-D) 6-furfurylamonopurine (KIN) or 1-naphthaleneacetic (NAA) 6-benzylaminopurine (BAP) establish phytohormones requirement promote best induction friable calli. For embryos induction, embryogenic calli axes, explants were cultured MS full strength MS/2, half strength, free media. This found that all exhibited high frequency callus induction. axes explants, media containing combination 2,4-D KIN effective callogenesis most important percentages obtained culture highest concentrations (2 mg L-1) (0.5 L-1). However, NAA BAP A maximal rate reached 3 L-1 1 BAP. number axis medium. About 56% converted successfully into fertile plantlets.